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CopR is one of the two components regulating replication of plasmid pIP501. It binds as a preformed dimer at the DNA major groove thereby repressing transcription of the essential repR-mRNA 10-20-fold. Previously, the DNA-binding motif was identified and the location of the dimeric interface was narrowed down. The C-terminal 29 residues were shown to be required exclusively for CopR stability. Here,...
The IncHI2 plasmid R478, like other arsenic resistance IncH plasmids, provides increased levels of resistance to sodium arsenate (up to 100mM) and sodium arsenite (up to 10mM) to the host cell. An arsenic resistance fragment of R478 was cloned and sequenced revealing four arsenic resistance associated gene homologues, arsR, arsB, arsC, and arsH. Two other open reading frames in the cloned fragment...
Two 11.8 kb non-conjugative, but mobilizable R plasmids designated pRAS3.1 and pRAS3.2 were isolated from Aeromonas salmonicida subspecies salmonicida and atypical A. salmonicida, respectively. Differences between the plasmids were of minor extent and they are considered as being variants of the same plasmid, pRAS3. The genes repA, repB, mobA, mobC, mobD, and mobE were organized similar to corresponding...
Lactobacillus fermentum KC5b, a strain originally isolated from the human vagina, contains a cryptic plasmid pKC5b. The sequence and genetic organization of the 4392-bp plasmid were determined. It contains two convergently oriented replicons, which are homologous to each other and to the stable replicon of the Enterococcus faecium plasmid pMBB1. The two replicons of pKC5b were used either individually...
Endoribonuclease III (RNase III) is known to participate in a number of RNA maturation and decay pathways. Previous research carried out on citQRP mRNA processing had provided strong evidence that RNase III exists in Lactococcus lactis and acts as a major endoribonuclease in the control of citQRP mRNA stability. The DNA coding (rnc) for the putative lactococcal RNase III was cloned by PCR technology...
Pichia etchellsii CBS2011 (synonym Debaryomyces etchellsii) is a non-killer yeast harbouring two cryptic linear cytoplasmic DNA-elements, pPE1A (6.7kb) and pPE1B (12.8kb). Cloning and complete sequencing of pPE1A revealed a 6749-bp element with a remarkably high A+T content of 77.6%. The termini of pPE1A were found to consist of inversely orientated identical nucleotide repetitions of 178bp, to which...
We have modified an E. coli-staphylococcal shuttle vector for use in the general cloning and expression of genes from pathogenic staphylococci in Staphylococcus carnosus. As S. carnosus is non-pathogenic, this expression system will facilitate the study of the roles of individual gene products in the disease process. To evaluate the use of this expression system, a DNA fragment containing the Staphylococcus...
Broad-host-range incompatibility group P (IncP) plasmids RK2 and R751 have 9-bp direct repeats (DR) of unknown function located between their kilC and kilE loci. The nucleotide sequences of the 9-bp repeats are different for RK2 (an IncPα group plasmid) and R751 (IncPβ group), but both DR regions are organized similarly, including an 11-bp spacer with identical 5 ' -CGCCA-3 ' cores...
Transcription initiated at the bacteriophage λp o promoter gives a short RNA, called oop RNA. Early studies led to a proposal that this transcript plays a role in the initiation of λ DNA replication. In fact, the p o promoter is located in the λ replication region and it was suggested that oop RNA may be a primer for replication proceeding leftward from oriλ. However, since in vitro...
A cryptic plasmid of Helicobacter pylori, pKU701 (accession number AB078638), was isolated and the complete nucleotide sequence was determined. No drug resistance properties were mediated by pKU701. The 2454b pKU701 sequence, which had a 38% content of G-C residues, generated one polypeptide from a single open reading frame (ORF1). Extensive sequence homology was evident between pKU701 and ORF1 of...
pHCM2 is a 106kbp cryptic plasmid harboured by Salmonella typhi CT18, originally isolated from a typhoid patient in Vietnam. The genome of S. typhi CT18, including pHCM2, has recently been completely sequenced and annotated. Bioinformatic analysis revealed that 57% of the coding sequences (CDSs) encoded on pHCM2 display over 97% DNA sequence identity to the virulence-associated plasmid of Yersinia...
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